Extended Depth of Field
نویسنده
چکیده
I n t roduc t ion The narrow depth of field of the light microscope is either an asset or a liability depend microscopist. Occasionally, by limiting the image to a narrow plane in the vertical axis, i observer’s attention by isolating structures in a thick sample, perm object while ignoring distracting elements abov cases, this feature is a liability when the object’s importance lies in its three-dimensional con portions of it are rendered invisible when they fall outside the depth of fi
منابع مشابه
Simultaneous quantitative depth mapping and extended depth of field for 4D microscopy through PSF engineering
An extended depth of field (EDF) microscope that allows for quantitative axial positioning has been constructed. Past work has shown that EDF microscopy allows for features in varying planes to appear sharply focused simultaneously, however an inherent consequence of this is that depth information is lost. Here, a specifically engineered phase plate is used to create a point spread function (PS...
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